Kuroda Lab Protocol

Embryology

  • Artificial and Natural Fertilization Word
  • Animal Cap Assay Word
  • Dissociated-and-reaggregated cell assay Word
  • Spemann's organizer transplantation Word
  • Keller Explant Word

Molecular

  • Plasmid Midi-Prep PDF
  • RT-PCR (cold-condition) Word
  • RT-PCR (hot-condition) HTML
  • Primers for RT-PCR Excel
  • Northern blot Word
  • Western blot for phospho sample Word
  • Antibody Stripping Word
  • HE staining for section Word
  • AP immunostaining Word
  • Section in situ hybridization Word
  • Whole mount in situ hybridization HTML
  • LacZ staining for section Word
  • BDA staining on section Word
  • In vitro translation Word

その他 (Japanese Only)

  • 人工授精と自然授精の方法 Google Doc
  • アニマルキャップアッセイ Google Doc
  • ツメガエルの餌の入手方法 HTML
  • カナダバルサムの溶解方法 HTML
  • Poly-HEMAプレートの作り方 Google Doc