Kuroda Lab Protocol

Time course of Xenopus Stages You can download PDF file if click above.

Embryology: Artificial and Natural Fertilization: word Animal Cap Assay: word Dissociated-and-reaggregated cell assay: word Spemann's organizer transplantation: word Keller Explant: word Molecular: Plasmid Midi-Prep: pdf RT-PCR (cold-condition): word RT-PCR (hot-condition): html Primers for RT-PCR: excel Northern blot: word Western blot for phospho sample: word Antibody Stripping: word HE staining for section: word AP immunostaining: word Section in situ hybridization: word Whole mount in situ hybridization: html LacZ staining for section: word BDA staining on section: word in vitro translation: word その他(Japanese Only): 人工授精と自然授精の方法: google document アニマルキャップアッセイ: google document ツメガエルの餌の入手方法: html カナダバルサムの溶解方法: html Poly-HEMAプレートの作り方: google doc